Exploring science is typically characterized by a lot of puzzles, frustrations or even failures. This weblog is mainly intended to record my working, thinking and knowledge acquisitions. I expect that some reflection would refresh my mind from time to time, and motivate me to move further, and hopefully give me a better view about even changing the landscape of bioinformatics.
You are welcome to leave some comments, good or bad, but hopefully something constructive. Enjoy your surfing!
"Thermo Fisher Scientific, the world leader in serving science, today announced the availability of the Thermo Scientific Pierce Peptide Retention Time Calibration Mixture for the prediction of peptide retention times on reversed-phase high-performance liquid chromatography (HPLC) columns. The convenient, ready-to-use Pierce® Peptide Retention Time Calibration Mixture contains 15 synthetic, heavy peptides mixed at an equimolar ratio to elute across the chromatographic gradient. It can be used with Thermo Scientific Pinpoint Software to predict peptide retention time from sequence alone, using hydrophobicity factors, or to predict peptide retention time between instrument platforms. The Pierce Peptide Retention Time Calibration Mixture streamlines the transition from qualitative protein discovery results to the development of targeted mass spectrometry (MS) assays on Thermo Scientific Triple Quadrupole, Orbitrap and Exactive Instruments and all other mass spectrometers. It also saves time in peptide purification by increasing the prediction efficiency of peptide retention profiles. The mixture is useful in evaluating different reversed-phase column and gradient options, monitoring for autosampler and HPLC column performance characteristics and normalizing results between experiments and over time."